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Annals of Intensive Care
Fig. 1 | Annals of Intensive Care

Fig. 1

From: Fluorescence imaging of ATP in neutrophils from patients with sepsis using organelle-localizable fluorescent chemosensors

Fig. 1

Confocal micrographs of human polymorphonuclear neutrophils (PMNs) stained with PMAP-1 and MitoAP-1. a ATP on the plasma membrane was stained with PMAP-1. The bright green fluorescence observed on the plasma membrane of PMNs resulted from PMAP-1 conjugation to ATP (×100 oil objective, NA 1.4). Scale bar 10 μm. b ATP in the mitochondria was stained with MitoAP-1 (red) and MitoTracker® Green FM (green), and they colocalized well (×100 oil objective, NA 1.4). Scale bar 10 μm

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