A novel sampling method to detect airborne influenza and other respiratory viruses in mechanically ventilated patients: a feasibility study

Annals of Intensive Care

Table 1 Virus inoculation and subsequent recovery by PCR

Virus	Treatment	Conditions	1 week	2 weeks	4 weeks
Influenza virus	Negative controls^a		No	No	No
	Virus added	20 °C	Detected	No	No
	Virus added	− 20 °C	Detected	Detected	Detected
Rhinovirus	Negative controls^a		No	No	No
	Virus added	20 °C	Detected	No	No
	Virus added	− 20 °C	Detected	Detected	Detected

10 uL of viral stock was inoculated onto each ventilator filter. These filters were then stored at either room temperature (20 °C) or low temperature (− 20 °C) for 1, 2 or 4 weeks. Triplicates were stored for each condition
^aNegative controls did not have any virus particles added to the filter