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Fig. 1 | Annals of Intensive Care

Fig. 1

From: Midazolam increases preload dependency during endotoxic shock in rabbits by affecting venous vascular tone

Fig. 1

Flowchart of the experiment protocol. After animal preparation (T0), an intravenous infusion of 0.5 mg kg−1 of LPS over 30 s was used to induce the endotoxic status, which was confirmed by a 25% decrease in mean arterial pressure. All animals received fluid resuscitation of 20 mL normal saline intravenously and then 50 mL kg−1 normal saline for another 2 h to maintain blood pressure. Norepinephrine infusion was initiated after fluid resuscitation and titrated to maintain the baseline blood pressure (T1). Midazolam was intravenously infused at doses of 0.3 or 3 mg kg−1 h−1 (T2). Equivalent normal saline was administered to rabbits in the Control group. A second round of fluid resuscitation and norepinephrine infusion was initiated to return the blood pressure back to normal (T3)

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