Fig. 2

Unbiased transcriptomic analysis reveals primary hemostasis-related genes as upregulated in critically ill COVID-19 patients. RNA from patients of Cohort 2 was extracted from whole blood and RNA counts of 594 genes were determined by direct probe hybridization using the Nanostring nCounter Human Immunology v2 kit. a Gene-set enrichment analysis was performed after ranking genes according to their differential expression in severe vs critical patients. Shown are pathways significantly enriched (false discovery rate < 0.2). b Heatmap representation of genes of the primary hemostasis pathway that are upregulated in a severity grade-dependent manner (determined by hierarchical clustering). Up-regulated genes are shown in red and down-regulated genes in blue. Groups: healthy controls (n = 13), mild-to-moderate (n = 11), severe (n = 10) and critical (n = 11)