Fig. 1

The Sanger sequencing method and next-generation sequencing steps. Sanger sequencing relies on dye-labeled nucleotides that are added to an elongating DNA strand thus determining each base according to the color of the dye. One of the NGS technique allows the specific amplification of an isolated DNA fragment by slide fixation such as in the Illumina chemistry, which is based on-chip amplification in bridge connection that allows simultaneous identification of DNA bases which emit a unique fluorescence signal when they are incorporated into the nucleic acid chain. PCR: polymerase chain reaction. NGS: next-generation sequencing