Fig. 4

Lymphocyte proliferation and IFN-γ production after rhIL-7 and TCR stimulation in severe COVID-19 patients. PBMCs were collected in COVID-19 patients at D0 (n = 10). Cells were cultured for 3 days in the presence or not of anti-CD2/CD3/CD28 Ab-coated beads (TCR Stimulation, ratio of beads/cells = 2:1) and rhIL-7 (TCR Stimulatio n + rhIL-7,100 ng/ml). Cellular proliferation was assessed by monitoring EdU AF488 incorporation into cells and expressed as percentages of cells incorporating EdU. IFN-γ was measured in cell supernatants after proliferation. Results are presented as individual values and medians. a CD4 + T cell proliferation is presented. b CD8 + T cell proliferation is presented. c Natural log transformed IFN-γ production in culture supernatants is shown. Wilcoxon signed-rank test was used to compare results between groups. Only statistically significant differences are shown